Osteogenic Differentiation of Dental Follicle Stem Cells

Osteogenic Differentiation of Dental Follicle Stem Cells

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Background: Stem cells are defined as clonogenic cells capable of self-renewal and multi-lineage differentiation.A population of these cells has been identified in human Dental Follicle (DF).Dental Follicle Stem Cells (DFSCs) were found in pediatric unerupted wisdom teeth and 6 LP Glides have been shown to differentiate, under particular conditions, into various cell types of the mesenchymal tissues.Aim: The aim of this study was to investigate if cells isolated from DF show stem features, differentiate toward osteoblastic phenotype and express osteoblastic markers.Methods: We studied the immunophenotype of DFSCs by flow cytometric analysis, the osteoblastic markers of differentiated DFSCs were assayed by histochemical methods and real-time PCR.

Results: We demonstrated that DFSCs expressed a heterogeneous assortment of makers associated with stemness.Moreover DFSCs differentiated into osteoblast-like cells, producing mineralized matrix nodules and expressed the typical osteoblastic markers, Alkaline Phosphatase (ALP) and Collagen I (Coll I).Conclusion: This study suggests that DFSCs may provide a cell source for tissue GANDALF SPIRULINA engineering of bone.